carborundum virus inoculation

Experimental Inoculation of Plants and Animals with Ebola

virus mixed with Carborundum powder was rubbed gently with cotton buds onto two leaves on each of the plant varieties on experiment; to simulate vector-borne trans-mission, a second plant of each type was inoculated with 1.0µl virus suspension into the phloem of the stem, using the same apparatus as for arthropods. A third plant of Table 1.

Potyvirus virion structure shows conserved protein fold

Sep 01, 2017· WMV inoculation and purifiion. Carborundum-dusted cotyledons of squash plants were inoculated with an homogenate consisting of a dried material from WMV-M116–infected squash leaves ground in 30 mM sodium phosphate (pH 8.0) . The inoculated plants were grown in a greenhouse (16-hour photoperiod; 25°/18°C day/night) for 4 weeks.

A Foxtail mosaic virus Vector for Virus-Induced Gene

Plant viruses have been widely used as vectors for foreign gene expression and virus-induced gene silencing ([VIGS][1]). A limited nuer of viruses have been developed into viral vectors for the purposes of gene expression or [VIGS][1] in monocotyledonous plants, and among these, the tripartite viruses Brome mosaic virus and Cucuer mosaic virus have been shown to induce [VIGS][1] in maize

Frontiers | dsRNA Molecules From the Tobacco Mosaic Virus

Exogenous appliion of double-stranded RNA (dsRNA) in the tobacco–Tobacco mosaic virus (TMV) pathosystem was shown previously to induce resistance against TMV providing an alternative approach to transgenesis. In the present study, we employed proteomics technology to elucidate the effect of TMV on tobacco as well as the effect of exogenous appliion of TMV p126 dsRNA molecules

Effectiveness of disinfectants against the spread of

Jan 06, 2021· Virus inoculum preparation and mechanical inoculation on test plants. The virus inoculum was prepared by grinding the symptomatic leaves (1:5 w/v) in plastic tissue extraction bags containing 1 × phosphate-buffered saline solution, pH 7.0 (140 mM NaCl, 8 mM Na 2 HPO 4, 1.5 mM KH 2 PO 4, 2.7 mM KCl, and 0.8 mM Na 2 SO 3) using a Homex-6 tissue

Cowpox - Wikipedia

After inoculation, vaccination using the cowpox virus became the primary defense against smallpox. After infection by the cowpox virus, the body (usually) gains the ability to recognize the similar smallpox virus from its antigens and is able to fight the smallpox disease much more efficiently.. The cowpox virus contains 186 thousand base pairs of DNA, which contains the information for about

Frontiers | dsRNA Molecules From the Tobacco Mosaic Virus

Exogenous appliion of double-stranded RNA (dsRNA) in the tobacco–Tobacco mosaic virus (TMV) pathosystem was shown previously to induce resistance against TMV providing an alternative approach to transgenesis. In the present study, we employed proteomics technology to elucidate the effect of TMV on tobacco as well as the effect of exogenous appliion of TMV p126 dsRNA molecules

OF TOBACCO MOSAIC VIRUS (l)

the virus particles penetrate the epidermal cells, the problem has not been completely solved (Herridge and Schlegel, 1962; Mun-dry, 1963; Brants, 1964, 1965, 1971; Gerola et al., 1969). It is known that rubbing the leaf surface with abrasives such as carborundum (Rawlings and Tompkins, 1934) and celite

Experimental Inoculation of Plants and Animals with Ebola

Dec 21, 2010· Experimental Inoculation of Plants and Animals with Ebola Virus. Peters CJ, Sanchez A, Rollin PE, Ksiazek TG, Murphy FA. Filoviridae: Marburg and Ebola viruses. In: Field''s Virology, Philadelphia: Lippincott-Raven. Le Guenno B, Formenty P, Wyers M, Gounon P, Walker F, Boesch C. Isolation and partial

Mechanism of the action of abrasives on infection by

With 600-mesh Carborundum powder in the inoculum at pH 8.5 and 0.1 M buffer, the greatest amount of infection occurred at 7% abrasive. With 10% abrasive in the inoculum, there was an increase in the amount of infection with a decrease in the particle size of the abrasive in the range of 150 to 800 mesh. The increase in the amount of infection due to abrasives of 240 and 800 mesh was related to the nuer of abrasive particles in the inoculum …

INTERACTION OF TOBACCO ETCH VIRUS AND THE ROOT …

Capsicum frutescens cv. tabasco with tobacco etch virus were made to insure purity and virulence of the virus. Virus inocula were obtained by grinding infected leaves in a mortar containing .01 M Na—KPti^ buffer pH 7.0 and 600 mesh carborundum. Inoculations were performed by dipping an acid brush into the inoculum and gently brushing

New Virus Complex of Chrysanthemums

oculation tests employing the carborundum technique, stunt appears inthe new growth three to four months after inoculation (Table 2). A three month period of waiting was also noted by Olson (1949) withthevariety Bronze Minuet. Thisperiod between inoculation and symptom expression is unusually long for a virus of herbaceous plants and constitutes

Wheat Cultivar-Specific Disease Synergism and Alteration

10 days before inoculation of experimental plants to obtain a fresh source of inoculum. Host range. WSMV or TriMV in crude sap at 1:20 dilution in 20 mM sodium phosphate buffer, pH 7.0 (inoculation buffer), was used to mechanically inoculate 15 to 35 carborundum-dusted seedlings that were 7 to 10 days old (Table 1). Inoculated plants

Coronavirus vaccine: New guidelines make inoculation more

Mar 26, 2021· Coronavirus vaccine: New guidelines make inoculation more accessible Some pharmacies and doctors'' offices may now store the vaccine …

Coronavirus: Hong Kong cancer patient given wrong vaccine

May 09, 2021· Coronavirus: Hong Kong cancer patient given wrong vaccine in mix-up over inoculation centres can have two BioNTech jabs Health authorities make an …

siRNA biogenesis and advances in topically applied dsRNA

Dec 17, 2020· We have also observed that co-appliion of dsRNA and virus is more efficient than pre-treating plants one day prior to virus inoculation (Fig. 5), indiing prompt protection.

Russia COVID-19 inoculation drive lags as vaccine

May 03, 2021· A woman receives a dose of Sputnik V (Gam-COVID-Vac) vaccine against the coronavirus disease (COVID-19) at a vaccination centre in a shopping mall in Saint Petersburg, Russia February 24, 2021.

New Virus Complex of Chrysanthemums

oculation tests employing the carborundum technique, stunt appears inthe new growth three to four months after inoculation (Table 2). A three month period of waiting was also noted by Olson (1949) withthevariety Bronze Minuet. Thisperiod between inoculation and symptom expression is unusually long for a virus of herbaceous plants and constitutes

Latent Period and Generation Time for Two Plant Viruses

after inoculation, the bean leaves were washed with 1 Received for publiion March 31, 1952. water to remove the carborundum, plant juice, virus, and phosphate from the plant surface. At 3 or 4 days after inoculation the nuer of local virus lesions on the test and control leaves was counted. Actual results of 4 trials are presented but most of

Inoculation - Wikipedia

Inoculation originated as a method for the prevention of smallpox by deliberate introduction of material from smallpox pustules into the skin. This generally produced a less severe infection than naturally acquired smallpox, but still induced immunity to it. This first method for smallpox prevention, smallpox inoculation, is now also known as variolation.

Frontiers | Cell Death Is Not Sufficient for the

Feb 15, 2018· Virus Spread Is Continuous in SA Depleted Transgenic Counterparts. NahG-Rywal transgenic plants are unable to restrict the virus close to the site of inoculation, which leads to the systemic spread of the virus (Baebler et al., 2014). Therefore, we investigated them in a similar setup to study the dynamics of virus spread around the lesions in

Systemic spread of an RNA insect virus in plants

Apr 24, 2001· Flock house virus (FHV), a single-stranded RNA insect virus, has previously been reported to cross the kingdom barrier and replie in barley protoplasts and in inoculated leaves of several plant species [Selling, B. H., Allison, R. F. & Kaesberg, P. (1990) Proc. Natl. Acad. Sci. USA 87, 434–438]. There was no systemic movement of FHV in plants. We tested the ability of movement …

Virus-induced down-regulation of GmERA1A and GmERA1B genes

Apr 18, 2017· Virus inoculation. C. quinoa and soybean plants were inoculated with ALSV as described [19, 23, 41]. The plasmids for ALSV-RNA1 and RNA2 were mixed in equal amounts, and the DNA solution was mechanically inoculated onto the true leaves of C. quinoa using carborundum. Reverse osmosis (RO) water was used for mock inoculation.

(PDF) Tobacco mosaic virus inoculation inhibits wound

Tobacco mosaic virus inoculation inhibits wound-induced jasmonic acid-mediated responses within but not between plants. Ian Baldwin. Related Papers. Nicotine and related alkaloids accumulation in constitutive salicylic acid producing tobacco plants. By Anjas Nugroho.

siRNA biogenesis and advances in topically applied dsRNA

Dec 17, 2020· We have also observed that co-appliion of dsRNA and virus is more efficient than pre-treating plants one day prior to virus inoculation (Fig. 5), indiing prompt protection.

Cuba begins mass Covid-19 vaccine inoculation before

May 13, 2021· Cuba began the mass inoculation of its population Wednesday with two homegrown Covid-19 vaccines that have yet to conclude late-stage clinical with limited flights, the virus …

Paenibacillus lentimorbus Inoculation Enhances Tobacco

Mar 02, 2016· A set of 10 plants mock inoculated with virus inoculation buffer was included in experiments as control. The suspension was squeezed through two layers of muslin cloth and sap obtained was mechanically rubbed on carborundum pre-dusted leaf of 3–4 leaf stage (four weeks old) tobacco seedlings. Severity of the disease symptom (mosaic, leaf